DNA

Part:BBa_K4162021:Design

Designed by: Weiwen Chen   Group: iGEM22_Fudan   (2022-10-05)


ribozyme+RBS+CDS module: crtIYEB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4479
    Illegal NgoMIV site found at 4609
    Illegal AgeI site found at 3637
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

crtIYEB separated by hammerhead ribozyme


Source

crtIYEB separated by hammerhead ribozyme

References